Coding

Part:BBa_K2578711:Design

Designed by: Yam Shing Fung   Group: iGEM18_Hong_Kong_JSS   (2018-09-14)


Protein coding sequence for Mouse metallothionein 1 (Mt1)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 374
    Illegal PstI site found at 639
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 639
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 218
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 374
    Illegal PstI site found at 639
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 374
    Illegal PstI site found at 639
    Illegal AgeI site found at 252
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The DNA sequence was modified to suit the synthesis by IDT while retaining the original amino acid sequence.


Source

NCBI

References

[1] https://www.ncbi.nlm.nih.gov/gene/17748

[2] N Thirumoorthy, KT Manisenthil Kumar, A Shyam Sundar, L Panayappan, Malay Chatterjee (2007). Metallothionein: An overview. World journal of gastroenterology, ISSN 1007-9327

[3] Almaguer-Cantú V1, Morales-Ramos LH, Balderas-Rentería I. (2011) Biosorption of lead (II) and cadmium (II) using Escherichia coli genetically engineered with mice metallothionein I. Water Sci Technol. 2011;63(8):1607-13.

[4] Dziegiel, P., Pula, B., Kobierzycki, C., Stasiolek, M., Podhorska-Okolow, M. (2016), Metallothioneins in Normal and Cancer Cells, Springer International Publishing, DOI: 10.1007/978-3-319-27472-0

[5] Almaguer-Cantú, V., Morales-Ramos, L. H., & Balderas-Rentería, I. (2011). Biosorption of lead (II) and cadmium (II) using Escherichia coli genetically engineered with mice metallothionein I. Water Science and Technology, 63(8), 1607-1613. doi:10.2166/wst.2011.225

[6] Jiang, Y., & Kang, Y. J. (2004). Metallothionein Gene Therapy for Chemical-Induced Liver Fibrosis in Mice. Molecular Therapy, 10(6), 1130-1139. doi:10.1016/j.ymthe.2004.08.011